Journal: PLOS One

Article Title: 3-O-acetylrubiarbonol B preferentially targets EGFR and MET over rubiarbonol B to inhibit NSCLC cell growth

doi: 10.1371/journal.pone.0329706

Figure Lengend Snippet: (A) Viability of NSCLC cells (HCC827 and HCC827GR) treated for 24 (black) and 48 h (white) with Ru-B (2, 4, 6, and 8 µM), ARu-B (2, 4, and 6 µM), gefitinib (GEF, 1 µM), and savolitinib (SAV, 2 nM) as monitored by the MTT assay. Data are shown as the mean ± SD (n = 3). (B) Cell viability of HEKa cells treated as in (A). (C) and (D) The soft agar assay was used to determine anchorage-independent colony growth in NSCLC cells (14 days of incubation). (C) Micrograph of the cells on day 14 and (D) colony number. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to vehicle only. Scale bar, 400 µm.

Article Snippet: HCC827 (GEF-sensitive NSCLC cell line) and HEKa (Human epidermal keratinocytes) cells were purchased from the ATCC (American Type Culture Collection, Manassas, USA).

Techniques: MTT Assay, Soft Agar Assay, Incubation

Journal: ACS Nano

Article Title: Multiplexed Nanoscale Viscoelastic Mapping at Multiple Time Scales of Melanoma Cells as a Label-Free Cancer Biomarker

doi: 10.1021/acsnano.5c01873

Figure Lengend Snippet: Altered organization of filamentous actin and microtubule cytoskeletons in melanoma cancer cells. Maximum intensity Z-projections (XY) demonstrating alterations in filamentous actin (magenta) and microtubule cytoskeletons (green). Immunofluorescence images of cells were captured using a Zeiss LSM 900 Airyscan 2. Compared with benign counterpart fibroblasts and melanocytes, highly malignant melanoma cells exhibit significant decreases in F-actin and microtubule network density.

Article Snippet: Human primary epidermal melanocyte cells were obtained from ATCC (Cat. # PCS-200-013) and cultured in Dermal Cell Basal Medium (ATCC, Cat. # PCS-200-030) supplemented with Phenol Red (ATCC) and Adult Melanocyte Growth Kit (ATCC, Cat. # PCS-200-042).

Techniques: Immunofluorescence